Recombinant Human Cereblon/DDB1/Cul4A/Rbx1 Complex Protein, His-tagged

• Cat.No.: CRBN-02H
• Product Overview: Cereblon/DDB1/Cul4A/Rbx1 complex of human Cereblon (GenBank Accession No. NM_016302) a.a. 2-442(end) with N-terminal FLAG-tag, human DDB1 (GenBank Accession No. NM_001923), a.a. 1-1140(end) with N-terminal FLAG-tag, human Cul4A (GenBank Accession No. NM_003589), a.a. 2-759(end) with N-terminal His-tag, human Rbx1 (GenBank Accession No. NM_014248), a.a. 2-108(end) with N-terminal His-tag, co-expressed in a HEK293 expression system.

Specification

• Description: This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Form: Aqueous buffer solution.
• Molecular Mass: Cereblon: 51 kDa; DDB1: 128 kDa; Cul4a: 88 kDa; Rbx1: 13 kDa
• Applications: Useful for the study of protein degradation and PROTAC design, and selectivity profiling.
• Storage: At least 6 months at –80 centigrade.
• Storage Buffer: 40 mm Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% Glycerol.
• Warning: Avoid multiple freeze/thaw cycles.

Gene Information

• Gene Name: CRBN cereblon [ Homo sapiens (human) ]
• Official Symbol: CRBN
• Synonyms: CRBN; cereblon; MRT2; MRT2A; protein cereblon; protein x 0001
• Gene ID: 51185
• mRNA Refseq: NM_016302
• Protein Refseq: NP_057386
• MIM: 609262
• UniProt ID: Q96SW2