Recombinant Full Length Human GSTA4 Protein, C-Flag-tagged

• Cat.No.: GSTA4-2071HFL
• Product Overview: Recombinant Full Length Human GSTA4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 25.5 kDa
• AA Sequence: MAARPKLHYPNGRGRMESVRWVLAA AGVEFDEEFLETKEQLYKLQDGNHL LFQQVPMVEIDGMKLVQTRS ILHYIADKHNLFGKNLKERTLIDMY VEGTLDLLELLIMHPFLKPDDQQKE VVNMAQKAIIRYFPVFEKIL RGHGQSFLVGNQLSLADVILLQTIL ALEEKIPNILSAFPFLQEYTVKLSN IPTIKRFLEPGSKKKPPPDE IYVRTVYNIFRP TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450

Gene Information

• Gene Name: GSTA4 glutathione S-transferase alpha 4 [ Homo sapiens (human) ]
• Official Symbol: GSTA4
• Synonyms: GTA4; GSTA4-4
• Gene ID: 2941
• mRNA Refseq: NM_001512.4
• Protein Refseq: NP_001503.1
• MIM: 605450
• UniProt ID: O15217