Recombinant Human Glutathione S-transferase Alpha 4, His-tagged

• Cat.No.: GSTA4-1956H
• Product Overview: Recombinanthuman GSTA4 protein, fused to His-tag at N-terminus, was expressed in E. coliand purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: GSTA4 belongs to the GST superfamily. Thisenzyme is involved in cellular defense against toxic, carcinogenic, andpharmacologically active electrophilic compounds. GSTA4 shows very highactivity with reactive carbonyl compounds such as alk-2-enals. GSTA4 ishighly effective in catalyzing the conjugate addition of reduced glutathioneto 4-hydroxynonenal, an important product of peroxidative degradation ofarachidonic acid and a commonly used biomarker for oxidative damage intissue. This enzyme is expressed at a high level inbrain, placenta, and skeletal muscle and much lower in lung and liver.
• Form: Liquid. In 20 mMTris-HCl buffer, pH 8.0, 20% glycerol, 2mM DTT, 100mM NaCl.
• Molecular Weight: 28.3 kDa (246aa)confirmed by MALDI-TOF
• Purity: > 95% by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQDGNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDDQQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTVKLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: GSTA4
• Pathway: Biological oxidations; Drugmetabolism-cytochrome P450; Glutathione conjugation; Glutathione metabolism;Metabolism; Metabolism of xenobiotics by cytochrome P450; Phase IIconjugation

Gene Information

• Gene Name: GSTA4glutathione S-transferase alpha 4 [ Homo sapiens ]
• Synonyms: GSTA4; glutathioneS-transferase alpha 4; GTA4; GSTA4-4; OTTHUMP00000016624; OTTHUMP00000016625;OTTHUMP00000016626; OTTHUMP00000016627; glutathione S-transferase A4; GlutathioneS-transferase A4-4; GST class-alpha member 4; EC 2.5.1.18; DKFZp686D21185; glutathioneS-alkyltransferase A4; glutathione S-aralkyltransferase A4; glutathioneS-aryltransferase A4; S-(hydroxyalkyl)glutathione lyase A4
• Gene ID: 2941
• mRNA Refseq: NM_004177
• Protein Refseq: NP_004168
• MIM: 605450
• UniProt ID: Q13277
• Chromosome Location: 6p12.1