Recombinant Full Length Human MAPK1 Protein, GST-tagged MAPK1-5665HF

Recombinant Full Length Human MAPK1 Protein, GST-tagged

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Recombinant Full Length Human MAPK1 Protein, GST-tagged

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Cat.No. : MAPK1-5665HF Optional Service: Optional requirements on this protein
Product Overview : Full-length human Erk2 [1-360(end) amino acids of accession number NP_002736.3] was expressed as N-terminal GST-fusion protein (69 kDa) using E.coli expression system. GST-Erk2 was purified by using glutathione sepharose chromatography and activated with His-tagged MAP2K1. Activated GST-Erk2 was purified using glutathione sepharose chromatography.
Source : E. coli
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Protein length : 1-360(end)
Purity : 94%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
SDS-PAGE :

The purity was assessed by SDS-PAGE/CBB staining.

Activity data :

The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.

Gene Name : mitogen-activated protein kinase 1
Official Symbol : MAPK1
Synonyms : MAPK1;ERK;ERK-2;ERK2;ERT1;MAPK2;P42MAPK;PRKM1;PRKM2;p38;p40;p41;p41mapk;p42-MAPK
Protein Refseq : NP_002736.3
UniProt ID : P28482
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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