Active Recombinant Full Length Human MAPK1 Protein, GST-tagged

• Cat.No.: MAPK1-5665HF
• Product Overview: Full-length human Erk2 [1-360(end) amino acids of accession number NP_002736.3] was expressed as N-terminal GST-fusion protein (69 kDa) using E.coli expression system. GST-Erk2 was purified by using glutathione sepharose chromatography and activated with His-tagged MAP2K1. Activated GST-Erk2 was purified using glutathione sepharose chromatography.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-360(end)
• Purity: 94%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase 1
• Official Symbol: MAPK1
• Synonyms: MAPK1;ERK;ERK-2;ERK2;ERT1;MAPK2;P42MAPK;PRKM1;PRKM2;p38;p40;p41;p41mapk;p42-MAPK
• Protein Refseq: NP_002736.3
• UniProt ID: P28482