Recombinant Full Length Human PLAU Protein, C-Flag-tagged

• Cat.No.: PLAU-1393HFL
• Product Overview: Recombinant Full Length Human PLAU Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.3 kDa
• AA Sequence: MRALLARLLLCVLVVSDSKGSNELH QVPSNCDCLNGGTCVSNKYFSNIHW CNCPKKFGGQHCEIDKSKTC YEGNGHFYRGKASTDTMGRPCLPWN SATVLQQTYHAHRSDALQLGLGKHN YCRNPDNRRRPWCYVQVGLK PLVQECMVHDCADGKKPSSPPEELK FQCGQKTLRPRFKIIGGEFTTIENQ PWFAAIYRRHRGGSVTYVCG GSLISPCWVISATHCFIDYPKKEDY IVYLGRSRLNSNTQGEMKFEVENLI LHKDYSADTLAHHNDIALLK IRSKEGRCAQPSRTIQTICLPSMYN DPQFGTSCEITGFGKENSTDYLYPE QLKMTVVKLISHRECQQPHY YGSEVTTKMLCAADPQWKTDSCQGD SGGPLVCSLQGRMTLTGIVSWGRGC ALKDKPGVYTRVSHFLPWIR
  SHTKEENGLALTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Complement and coagulation cascades

Gene Information

• Gene Name: PLAU plasminogen activator, urokinase [ Homo sapiens (human) ]
• Official Symbol: PLAU
• Synonyms: ATF; QPD; UPA; URK; u-PA; BDPLT5
• Gene ID: 5328
• mRNA Refseq: NM_002658.6
• Protein Refseq: NP_002649.2
• MIM: 191840
• UniProt ID: P00749