| Species : |
Human |
| Source : |
E.coli |
| Protein Length : |
1-76aa |
| Description : |
Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways; it can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. Ubiquitin- Aldehyde is a potent, irreversible, and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Aldehyde group. It targets three of the four major DUB families: UCH (Ubiquitin c-terminal hydrolases), USP (Ubiquitin Specific Proteases), OTU (Ovarian Tumor Proteases), and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. |
| Conjugation/Label : |
Aldehyde |
| Molecular Mass : |
8.6 kDa |
| Purity : |
>97% by LCMS |
| Usage : |
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Aldehyde concentration of 1-5 μM. |
| Applications : |
Ub-Aldehyde can be used for activity profiling experiments and determining DUB inhibitor specificity. |
| Notes : |
For Research Use Only, Not For Use In Humans. |
| Storage : |
Store at −80 centigrade after product arrival. Avoid multiple freeze/thaws. It is recommended to make multiple aliquots after the first thaw. |
| Storage Buffer : |
50mM NaOAc pH 5.0 |
| Concentration : |
350uM, 3.1 mg/mL |
| Substrate Properties : |
Protein-Based Substrate. Typical experimental concentration 1-5 μM. |
