Recombinant Full Length Human Ubiquitin Protein, GST tagged

• Cat.No.: Ubiquitin-18HFL
• Product Overview: Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. This product can be used for pulldwon assay to determine ubiquitin-binding proteins.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Molecular Mass: 8.5 kDa
• Purity: ~90% by SDS-PAGE, see datasheet for gel image
• Storage: Store at -80 centigrade; avoid multiple freeze-thaw cycles
• Storage Buffer: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
• Shipping: Dry ice
• Reference: 1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786. 2. Pickart CM (1997) FASEB 11(13), 1055 – 1066. 3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.

Gene Information

• Official Symbol: Ubiquitin