Recombinant Human ABI2 293 Cell Lysate
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Cat.No. : | ABI2-9127HCL Optional Service: Optional requirements on this protein |
Description : | Antigen standard for abl-interactor 2 (ABI2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ABI2 abl-interactor 2 [ Homo sapiens ] |
Official Symbol : | ABI2 |
Synonyms : | ABI2; abl-interactor 2; abl interactor 2; ABI 2; ABI2B; AblBP3; AIP 1; argBPIA; SSH3BP2; argBP1; abelson interactor 2; abl binding protein 3; abl-binding protein 3; arg-binding protein 1; abl-interactor protein 2b; arg protein tyrosine kinase-binding protein; abl-interacting protein 1 (SH3-containing protein); ABI-2; AIP-1; argBPIB; |
Gene ID : | 10152 |
mRNA Refseq : | NM_005759 |
Protein Refseq : | NP_005750 |
MIM : | 606442 |
UniProt ID : | Q9NYB9 |
Chromosome Location : | 2q33 |
Pathway : | RAC1 signaling pathway, organism-specific biosystem; Regulation of Actin Cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem; |
Function : | DNA binding; SH3 domain binding; SH3 domain binding; cytoskeletal adaptor activity; kinase binding; proline-rich region binding; ubiquitin protein ligase binding; |
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