Recombinant Human Active IL23A Protein, His-tagged IL23A-02H

Recombinant Human Active IL23A Protein, His-tagged

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Cat.No. : IL23A-02H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human Active IL23A Protein with a His tag (N-ter) was expressed in E. coli.
Description : This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.
Source : E. coli
Species : Human
Tag : His
Form : Powder
Bio-activity : Measured by its ability to induce IL-17 secretion in mouse splenocytes. The ED50 for this effect is < 0.5 ng/mL
AA Sequence : RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP with polyhistidine tag at the N-terminus.
Endotoxin : < 0.1 EU/μg of the protein, as determined by the LAL test.
Purity : > 95 % by SDS-PAGE
Storage : Lyophilized protein should be stored at -20 centigrade. After reconstitution, aliquot and store at -20 or -80 centigrade for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Storage Buffer : PBS (pH 8.0)
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
SDS-PAGE :

SDS-PAGE analysis of Human IL23A / IL23 p19 recombinant protein (Active) (His-tagged, N-ter).

Gene Name : IL23A interleukin 23 subunit alpha [ Homo sapiens (human) ]
Official Symbol : IL23A
Synonyms : IL23A; interleukin 23 subunit alpha; P19; SGRF; IL-23; IL-23A; IL23P19; interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; IL-23p19; JKA3 induced upon T-cell activation; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit p19; interleukin-six, G-CSF related factor
Gene ID : 51561
mRNA Refseq : NM_016584
Protein Refseq : NP_057668
MIM : 605580
UniProt ID : Q9NPF7
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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