Active Recombinant Full Length Human AKT1 protein, His-tagged

• Cat.No.: AKT1-786H
• Product Overview: Active Recombinant Full Length Human AKT1 protein(NP_001014431.1) (Met 1-Ala 480) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-480 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 3mM DTT, 0.5mM PMSF, 5% Glycerol, pH 8.0.
• Bio-activity: 1. No Kinase Activity 2. Measured by its ability to bind biotinylated human CD136 in a functional ELISA.
• Molecular Mass: The recombinant human AKT1 consists of 491 amino acids and migrates as an approximately 57 kDa band as predicted in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 87 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: AKT1 v-akt murine thymoma viral oncogene homolog 1 [ Homo sapiens ]
• Official Symbol: AKT1
• Synonyms: AKT1; v-akt murine thymoma viral oncogene homolog 1; RAC-alpha serine/threonine-protein kinase; AKT; PKB; PRKBA; RAC; PKB alpha; RAC-PK-alpha; proto-oncogene c-Akt; protein kinase B alpha; rac protein kinase alpha; PKB-ALPHA; RAC-ALPHA; MGC99656
• Gene ID: 207
• mRNA Refseq: NM_001014431
• Protein Refseq: NP_001014431
• MIM: 164730
• UniProt ID: P31749