Recombinant Human ALKBH2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ALKBH2-5295H
• Product Overview: ALKBH2 MS Standard C13 and N15-labeled recombinant protein (NP_001138846) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 29.3 kDa
• AA Sequence: MDRFLVKGAQGGLLRKQEEQEPTGE EPAVLGGDKESTRKRPRREAPGNGG HSAGPSWRHIRAEGLDCSYTVLFGK AEADEIFQELEKEVEYFTGALARVQ VFGKWHSVPRKQATYGDAGLTYTFS GLTLSPKPWIPVLERIRDHVSGVTG QTFNFVLINRYKDGCDHIGEHRDDE RELAPGSPIASVSFGACRDFVFRHK DSRGKSPSRRVAVVRLPLAHGSLLM MNHPTNTHWYHSLPVRKKVLAPRVN LTFRKILLTKKTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ALKBH2 alkB homolog 2, alpha-ketoglutarate dependent dioxygenase [ Homo sapiens (human) ]
• Official Symbol: ALKBH2
• Synonyms: ALKBH2; alkB, alkylation repair homolog 2 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; ABH2; MGC90512; oxy DC1; 2OG-Fe(II) oxy DC1; alkylated DNA repair protein alkB homolog 2; FLJ99103
• Gene ID: 121642
• mRNA Refseq: NM_001145374
• Protein Refseq: NP_001138846
• MIM: 610602
• UniProt ID: Q6NS38