Recombinant Human ALOX15B Protein, His-tagged

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Recombinant Human ALOX15B Protein, His-tagged

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Cat.No. : ALOX15B-054H
Product Overview : Active human recombinant C-terminal His-tagged human ALOX15B protein was expressed in E. coli.
Description : This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 76 kDa
Purity : ≥85% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 nmol of 15-HpETE per min at 30 centigrade in 50mM Tris-HCl, pH7.2, with 0.003% Polysorbate 20, and 250 μM arachidonic acid.
Stability : ≥ 1 year
Storage : At -80 centigrade.
SDS-PAGE analysis of 15-LO-2 :

Lane 1: MW Markers
Lane 2: 15-LO-2 (human, recombinant) (1 μg)
Lane 3: 15-LO-2 (human, recombinant) (2 μg)

15-LO-2 Activity :

15-LO-2 activity was determined using 50 μM arachidonic acid and was inhibited using 100 μM NDGA, a non-selective lipoxygenase inhibitor.

Gene Name : ALOX15B arachidonate 15-lipoxygenase type B [ Homo sapiens (human) ]
Official Symbol : ALOX15B
Synonyms : ALOX15B; arachidonate 15-lipoxygenase type B; 15-LOX-2; polyunsaturated fatty acid lipoxygenase ALOX15B; 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate omega(6) lipoxygenase; linoleate 13-lipoxygenase 15-LOb; EC 1.13.11.33
Gene ID : 247
mRNA Refseq : NM_001141
Protein Refseq : NP_001132
MIM : 603697
UniProt ID : O15296
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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