Active Recombinant Full Length Human CA2 Protein, His tagged

• Cat.No.: CA2-114H
• Product Overview: Carbonic Anhydrase 2 Protein, Human (HEK293, His) is the recombinant human-derived Carbonic Anhydrase 2 protein, expressed by HEK293, with C-His tag. The total length of Carbonic Anhydrase 2 Protein, Human (HEK293, His) is 260 a.a., with molecular weight of approximately 31.72 kDa.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-260 aa
• Description: Carbonic Anhydrase 2 (CA2) catalyzes the reversible hydration of carbon dioxide. Mutations in CA2 are linked to osteopetrosis and renal tubular acidosis. The gene exhibits biased expression in various tissues, notably in the stomach and colon. Two transcript variants, encoding different isoforms, have been identified.
• Form: Lyophilized powder
• Molecular Mass: 31.72 kDa
• Bio-activity: Measured by its esterase activity. The specific activity is 1454.8 pmol/min/μg
• AA Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK
• Endotoxin: <1 EU/μg determined by LAL method.
• Purity: ≥98.0%
• Storage: Stored at -20 centigrade for 2 years from date of receipt. After reconstitution, it is stable at 4 centigrade for 1 week or -20 centigrade for longer (with carrier protein). It is recommended to freeze aliquots at -20 or -80 centigrade for extended storage.
• Storage Buffer: Lyophilized a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0 or 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
• Shipping: Room temperature in continental US; may vary elsewhere.

Gene Information

• Gene Name: CA2 carbonic anhydrase II [ Homo sapiens (human) ]
• Official Symbol: CA2
• Synonyms: CA2; carbonic anhydrase II; carbonic anhydrase 2; CA II; CAII; Car2; CAC; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; CA-II
• Gene ID: 760
• mRNA Refseq: NM_000067
• Protein Refseq: NP_000058
• MIM: 611492
• UniProt ID: P00918