|Product Overview :||Recombinant Human CD27 protein (Thr21-Ile192), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27/TNFRSF7 is found in most T-lymphocytes. CD27/TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.|
|Form :||Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 21.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||172|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CD27-336H :||
Human CD27 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of CD27-336H :||
Immobilized Human CD27, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Avitag,Fc Tag with a linear range of 2-31 ng/mL (QC tested).
|Gene Name :||CD27|
|Official Symbol :||CD27|
|Synonyms :||CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393|
|Gene ID :||939|
|mRNA Refseq :||NM_001242|
|Protein Refseq :||NP_001233|
|UniProt ID :||P26842|