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Recombinant Human CD33 protein, hFc-tagged

Cat.No. : CD33-345H
Product Overview : Recombinant Human CD33 protein(P20138-1)(Asp18-His259), fused with C-terminal hFc tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : C-hFc
Protein length : Asp18-His259
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human Siglec-3, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA.
Molecular Mass : The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Name : CD33 CD33 molecule [ Homo sapiens ]
Official Symbol : CD33
Synonyms : CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3;
Gene ID : 945
mRNA Refseq : NM_001082618
Protein Refseq : NP_001076087
MIM : 159590
UniProt ID : P20138

Not For Human Consumption!

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Customer Reviews (3)

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Reviews
11/14/2019

    Quick dissolution, easy use.

    08/27/2019

      Excellent solubility, no aggregates.

      02/27/2019

        High concentration, very effective.

        Q&As (7)

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        What are the implications of CD33 polymorphisms in therapeutic targeting? 10/18/2022

        Certain polymorphisms in the CD33 gene can influence its expression on the cell surface, which might affect the efficacy of CD33-targeted therapies.

        What is the primary biological function of CD33 in hematopoietic cells? 10/08/2022

        CD33 is a transmembrane receptor primarily expressed on cells of myeloid lineage and is involved in modulating cellular functions, including the regulation of cellular proliferation and differentiation.

        How does CD33 expression vary among different leukocyte populations? 01/11/2022

        CD33 is predominantly expressed on myeloid cells, especially on immature myeloid cells. Its expression decreases as these cells differentiate and is typically low or absent on lymphoid cells.

        How has CD33 been utilized as a therapeutic target in hematological malignancies? 09/22/2021

        CD33 has been targeted for therapeutic antibodies in AML. An example is gemtuzumab ozogamicin, which targets CD33 and delivers a cytotoxic agent specifically to leukemia cells expressing CD33.

        How does CD33 signaling influence myeloid cell differentiation? 06/08/2020

        While the exact signaling pathways of CD33 are still under investigation, it is believed that CD33 signaling can influence the differentiation and function of myeloid cells, potentially playing a role in immune regulation.

        What role does CD33 play in acute myeloid leukemia (AML)? 03/30/2019

        CD33 is commonly expressed on the surface of AML blasts, making it a potential target for therapeutic interventions in AML.

        Are there known ligands that bind specifically to CD33? 06/20/2018

        The specific ligands for CD33 are not fully characterized, but it is believed to play a role in cell-cell interactions within the immune system.

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