Recombinant Human CLEC12A protein, His,Avi-tagged, Biotinylated

• Cat.No.: CLEC12A-2948H
• Product Overview: Biotinylated Recombinant Human CLEC12A protein(Q5QGZ9-2)(His65-Ala265), fused with N-terminal His,C-terminal Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His,C-Avi
• Protein length: His65-Ala265
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human CLEC12A, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CLEC12A Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CLEC12A C-type lectin domain family 12, member A [ Homo sapiens ]
• Official Symbol: CLEC12A
• Synonyms: CLEC12A; C-type lectin domain family 12, member A; C-type lectin domain family 12 member A; CLL 1; MICL; C-type lectin superfamily; C-type lectin protein CLL-1; C-type lectin-like molecule-1; dendritic cell-associated lectin 2; myeloid inhibitory C-type lectin-like receptor; CLL1; CLL-1; DCAL-2; MGC70602
• Gene ID: 160364
• mRNA Refseq: NM_001207010
• Protein Refseq: NP_001193939
• MIM: 612088
• UniProt ID: Q5QGZ9