Recombinant Human CX3CL1 protein, His-tagged CX3CL1-399H

Recombinant Human CX3CL1 protein, His-tagged

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Recombinant Human CX3CL1 protein, His-tagged

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Cat.No. : CX3CL1-399H
Product Overview : Recombinant Human CX3CL1 protein (Gln25-Gln341), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Fractalkine is also known as C-X3-C motif chemokine 1 (CX3CL1) and neurotactin. Fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Fractalkine is found commonly throughout the brain, particularly in neural cells, and its receptor is known to be present on microglial cells. It has also been found to be essential for microglial cell migration. CX3CL1 is also up-regulated in the hippocampus during a brief temporal window following spatial learning, the purpose of which may be to regulate glutamate-mediated neurotransmission tone. This indicates a possible role for the chemokine in the protective plasticity process of synaptic scaling.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 35.5 kDa. The protein migrates as 50-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 317
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CX3CL1-399H :

Human CX3CL1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CX3CL1
Official Symbol : CX3CL1
Synonyms : CX3CL1; chemokine (C-X3-C motif) ligand 1; SCYD1, small inducible cytokine subfamily D (Cys X3 Cys), member 1 (fractalkine, neurotactin); fractalkine; ABCD 3; C3Xkine; CXC3; CXC3C; neurotactin; NTN; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); NTT; SCYD1; ABCD-3
Gene ID : 6376
mRNA Refseq : NM_002996
Protein Refseq : NP_002987
MIM : 601880
UniProt ID : P78423
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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