"EED" Related Products

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Recombinant Human EED protein, His-tagged

Cat.No.: EED-316H
Product Overview: Recombinant full-length human EED was expressed in E.coli cells using an N-terminal His tag.
Description: The embryonic ectoderm development protein (EED), a WD40 domain-containing protein, is the regulatory subunit of polycomb repressive complex 2 (PRC2). PRC2 catalyzes the monomethylation, dimethylation, and trimethylation of histone H3 Lys27 (H3K27) and acts as a central epigenetic regulator that marks the repressive chromatin domain. EED binds to the tri-methylated lysine 27 of the histone H3 (H3K27me3), and through which stimulates the activity of PRC2 allosterically.
Source: E.coli
Species: Human
Tag : His
Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Molecular Mass: 57 kDa
Purity: The purity of EED was determined to be >90% by densitometry.
Applications: Enzyme Assay; WB
Stability: 1 year at -70 centigrade
Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1 μg/μL
Gene Name: embryonic ectoderm development [ Homo sapiens (human) ]
Official Symbol: EED
Synonyms: HEED; WAIT1
Gene ID: 8726
mRNA Refseq: NM_003797
Protein Refseq: NP_003788
MIM: 605984
UniProt ID: O75530

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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