Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Description : |
The embryonic ectoderm development protein (EED), a WD40 domain-containing protein, is the regulatory subunit of polycomb repressive complex 2 (PRC2). PRC2 catalyzes the monomethylation, dimethylation, and trimethylation of histone H3 Lys27 (H3K27) and acts as a central epigenetic regulator that marks the repressive chromatin domain. EED binds to the tri-methylated lysine 27 of the histone H3 (H3K27me3), and through which stimulates the activity of PRC2 allosterically. |
Form : |
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol |
Molecular Mass : |
57 kDa |
Purity : |
The purity of EED was determined to be >90% by densitometry. |
Applications : |
Enzyme Assay; WB |
Stability : |
1 year at -70 centigrade |
Storage : |
Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : |
0.1 μg/μL |