Cat.No.:	EED-316H
Product Overview:	Recombinant full-length human EED was expressed in E.coli cells using an N-terminal His tag.
Description:	The embryonic ectoderm development protein (EED), a WD40 domain-containing protein, is the regulatory subunit of polycomb repressive complex 2 (PRC2). PRC2 catalyzes the monomethylation, dimethylation, and trimethylation of histone H3 Lys27 (H3K27) and acts as a central epigenetic regulator that marks the repressive chromatin domain. EED binds to the tri-methylated lysine 27 of the histone H3 (H3K27me3), and through which stimulates the activity of PRC2 allosterically.
Source:	E.coli
Species:	Human
Tag :	His
Form:	50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Molecular Mass:	57 kDa
Purity:	The purity of EED was determined to be >90% by densitometry.
Applications:	Enzyme Assay; WB
Stability:	1 year at -70 centigrade
Storage:	Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:	0.1 μg/μL

Gene Name:	embryonic ectoderm development [ Homo sapiens (human) ]
Official Symbol:	EED
Synonyms:	HEED; WAIT1
Gene ID:	8726
mRNA Refseq:	NM_003797
Protein Refseq:	NP_003788
MIM:	605984
UniProt ID:	O75530

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