Recombinant Human EFNB2 protein(Met1-Ala229), His-tagged

Cat.No. : EFNB2-3191H
Product Overview : Recombinant Human EFNB2 (NP_004084.1) (Met 1-Ala 229) was expressed in HEK293, fused with a C-terminal polyhistidine tag.
Availability July 20, 2024
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Source : HEK293
Species : Human
Tag : His
Protein length : Met1-Ala229
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized human EFNB2 at 2 μg/ml (100 μl/well) can bind human EphB4 with a linear range of 1.56-12.5 ng/ml.
Molecular Mass : The recombinant human EFNB2 consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB2 is approximately 35-40 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 97 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : EFNB2 ephrin-B2 [ Homo sapiens ]
Official Symbol : EFNB2
Synonyms : EFNB2; ephrin-B2; EPLG5; eph related receptor tyrosine kinase ligand 5; HTK ligand; Htk L; HTKL; LERK5; ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; LERK-5; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5; Htk-L;
Gene ID : 1948
mRNA Refseq : NM_004093
Protein Refseq : NP_004084
MIM : 600527
UniProt ID : P52799

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
01/05/2022

    During the experiment, the technician helped me solve the problems in a timely and accurate manner.

    01/02/2022

      The EFNB2 proteins purchased from your company is suitable for WB.

      01/23/2021

        Received high quality protein products and obtained satisfactory experimental results.

        Q&As (6)

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        What effect does EphB6 overexpression have on EFNB2? 05/06/2022

        EphB6 overexpression up-regulates the expression of its ligand EFNB2.

        What diseases is EFNB2 involved in? 01/02/2022

        EFNB2 is associated with breast, colon, bladder, cervical and other cancers.

        What is the biological function of EFNB2? 05/05/2021

        EFNB2 is involved in tissue plasticity, tumorigenesis, and other biological processes.

        Where is EFNB2 mainly expressed? 04/10/2021

        EFNB2 is widely expressed in tumor vasculature.

        What is the association between EFNB2 and BUC cells? 03/12/2021

        EFNB2 promotes BUC cell proliferation by inhibiting apoptosis in BUC cells.

        How is EphB6 related to EFNB2? 07/09/2019

        EFNB2 is one of the physiological ligands of EphB6 that functions by binding to EphB6.

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