Recombinant Rat EFNB2 cell lysate

• Cat.No.: EFNB2-1540RCL
• Product Overview: Rat EFNB2 / EphrinB2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Rat
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Efnb2 ephrin B2 [ Rattus norvegicus ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin B2
• Gene ID: 306636
• mRNA Refseq: NM_001107328
• Protein Refseq: NP_001100798
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: ephrin receptor binding; ephrin receptor binding; receptor binding

Reviews

01/05/2022

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

01/02/2022

The EFNB2 proteins purchased from your company is suitable for WB.

01/23/2021

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What effect does EphB6 overexpression have on EFNB2?

EphB6 overexpression up-regulates the expression of its ligand EFNB2.

What diseases is EFNB2 involved in?

EFNB2 is associated with breast, colon, bladder, cervical and other cancers.

What is the biological function of EFNB2?

EFNB2 is involved in tissue plasticity, tumorigenesis, and other biological processes.

Where is EFNB2 mainly expressed?

EFNB2 is widely expressed in tumor vasculature.

What is the association between EFNB2 and BUC cells?

EFNB2 promotes BUC cell proliferation by inhibiting apoptosis in BUC cells.

How is EphB6 related to EFNB2?

EFNB2 is one of the physiological ligands of EphB6 that functions by binding to EphB6.