Active Recombinant Human EGFR protein, Fc/Avi-tagged, Biotinylated

• Cat.No.: EGFR-053H
• Product Overview: Biotinylated Recombinant Human EGFR(Leu25-Ser378) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells .

Specification

• Description: Epidermal growth factor receptor (EGFR), also known as HER-1 and ErbB1, is a member of a subfamily of receptor tyrosine kinases comprised of four members:
  EGFR, ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD)
  containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine
  autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3-4). EGFRvIII is a tumor‐specific
  mutation that results from an in‐frame deletion removing 267 amino acids from the ECD and insertion of a glycine residue (5). EGFRvIII has a molecular mass of
  approximately 145 kDa and has been shown to have weaker activity than full-length EGFR (6). EGFR binds a subset of the EGF family ligands, including EGF,
  amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2,
  resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-9). EGFR can also be recruited to form heterodimers with the ligandactivated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (7-9). EGFR is
  overexpressed in a wide variety of tumors, with EGFRvIII overexpressed particularly in glioblastoma multiforme (GMB), and is the target of several anti-cancer
  therapeutics (5,10,11). Our Avi-tag Biotinylated Recombinant Human EGFRvIII features biotinylation at a single site contained within the Avi-tag, a unique 15 amino
  acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is
  unchanged so there is no interference in the protein's bioactivity.
• Source: CHO cells
• Species: Human
• Tag: Fc/Avi
• Predicted N Terminal: Leu25
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.
  When Human EGFR antibody is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant
  Human EGFR Fc Chimera Avi-tag binds with an ED50 of 25-150 ng/mL.
• Molecular Mass: 95-115 kDa, under reducing conditions
• Protein length: Leu25-Ser378
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533