Recombinant Human ERCC3 protein, His & T7-tagged

• Cat.No.: ERCC3-1692H
• Product Overview: Recombinant Human ERCC3 aa. (Arg542~Ala702 (Accession # P19447)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.3kDa
• Protein length: Arg542~Ala702 (Accession # P19447)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 9.5

Gene Information

• Gene Name: ERCC3 ERCC excision repair 3, TFIIH core complex helicase subunit [ Homo sapiens (human) ]
• Official Symbol: ERCC3
• Synonyms: ERCC3; ERCC excision repair 3, TFIIH core complex helicase subunit; XPB; BTF2; TTD2; GTF2H; RAD25; TFIIH; TFIIH basal transcription factor complex helicase XPB subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair helicase; DNA repair protein complementing XP-B cells; TFIIH 89 kDa subunit; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH p89; basic transcription factor 2 89 kDa subunit; excision repair cross-complementation group 3; excision repair cross-complementing rodent repair deficiency, complementation group 3; xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B
• Gene ID: 2071
• mRNA Refseq: NM_000122.1
• Protein Refseq: NP_000113.1
• UniProt ID: P19447