Recombinant Human Eukaryotic Elongation Factor-2 Kinase, GST-tagged

• Cat.No.: EEF2K-1879H
• Product Overview: Recombinant humanfull length EEF2K GST-Tagged.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: EEF2K is a highlyconserved protein kinase in the calmodulin-mediated signaling pathwaythat links activation of cell surface receptors to cell division. This kinaseis involved in the regulation of protein synthesis. It phosphorylateseukaryotic elongation factor 2 and thus inhibits the EEF2 function. Theactivity of this kinase is increased in many cancers and may be a validtarget for anti-cancer treatment.
• Form: Liquid in 50 mMTris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mM DTT +50% glycerol.
• Molecular Weight: 109.8 kDa calculatedfrom the amino acid sequence below.
• Amino Acid Sequence: MSPILGYWKIKGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHNMLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTHPDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSDLVPRPWSNQT SLYKKAGTMA DEDLIFRLEG VDGGQSPRAG HDGDSDGDSD DEEGYFICPI TDDPSSNQNVNSKVNKYYSN LTKSERYSSS GSPANSFHFK EAWKHAIQKA KHMPDPWAEF HLEDIATERA TRHRYNAVTGEWLDDEVLIK MASQPFGRGA MRECFRTKKL SNFLHAQQWK GASNYVAKRY IEPVDRDVYF EDVRLQMEAKLWGEEYNRHK PPKQVDIMQM CIIELKDRPG KPLFHLEHYI EGKYIKYNSN SGFVRDDNIR LTPQAFSHFTFERSGHQLIV VDIQGVGDLY TDPQIHTETG TDFGDGNLGV RGMALFFYSH ACNRICESMG LAPFDLSPRERDAVNQNTKL LQSAKTILRG TEEKCGSPRV RTLSGSRPPL LRPLSENSGD ENMSDVTFDS LPSSPSSATPHSQKLDHLHW PVFSDLDNMA SRDHDHLDNH RESENSGDSG YPSEKRGELD DPEPREHGHS YSNRKYESDEDSLGSSGRVC VEKWNLLNSS RLHLPRASAV ALEVQRLNAL DLEKKIGKSI LGKVHLAMVR YHEGGRFCEKGEEWDQESAV FHLEHAANLG ELEAIVGLGL MYSQLPHHIL ADVSLKETEE NKTKGFDYLL KAAEAGDRQSMILVARAFDS GQNLSPDRCQ DWLEALHWYN TALEMTDCDE GGEYDGMQDE PRYMMLAREA EMLFTGGYGLEKDPQRSGDL YTQAAEAAME AMKGRLANQY YQKAEEAWAQ MEE
• Concentration: 0.43 mg/ml
• Purity: ≥ 75% as determinedby SDS-PAGE analysis.
• Specific Activity: 148 nmole ofphosphate transferred to EF2tide (RKKFGESEKTKTKEFL) per min per mg of totalprotein at 30°C. Activity determined at a final protein concentration of 3.33μg/ml.
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: EEF2K
• Full Length: Full L.

Gene Information

• Gene Name: EEF2K eukaryotic elongationfactor-2 kinase [ Homo sapiens ]
• Synonyms: EEF2K; eukaryoticelongation factor-2 kinase; eEF-2K; HSU93850; MGC45041; eukaryoticelongation factor 2 kinase; eEF-2 kinase; OTTHUMP00000119998; elongationfactor-2 kinase; eukaroytic elongation factor 2 kinase; calmodulin-dependentprotein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor2 kinase
• Gene ID: 29904
• mRNA Refseq: NM_013302
• Protein Refseq: NP_037434
• MIM: 606968
• UniProt ID: O00418
• Chromosome Location: 16p12
• Pathway: IRS-mediatedsignalling; IRS-related events; Insulin receptor signalling cascade; PI3KCascade; PKB-mediated events; S6K1 signalling; S6K1-mediated signalling;Signal Transduction; Signaling by Insulin receptor; Signaling mediated byp38-gamma and p38-delta; Translation Factors; mTOR signaling pathway; mTORsignalling; mTORC1-mediated signalling
• Function: ATP binding; calciumion binding; calmodulin binding; elongation factor-2 kinase activity;nucleotide binding; protein kinase activity; protein serine/threonine kinaseactivity; translation factor activity, nucleic acid binding