Recombinant Mouse Eef2k Protein, Myc/DDK-tagged

• Cat.No.: Eef2k-2739M
• Product Overview: Purified recombinant protein of mouse full-length eukaryotic elongation factor-2 kinase (Eef2k), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
• Molecular Mass: 82.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Eef2k eukaryotic elongation factor-2 kinase [ Mus musculus (house mouse) ]
• Official Symbol: Eef2k
• Synonyms: EEF2K; eukaryotic elongation factor-2 kinase; eukaryotic elongation factor 2 kinase; eEF-2 kinase; eukaroytic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; C86191; eEF-2K
• Gene ID: 13631
• mRNA Refseq: NM_007908
• Protein Refseq: NP_031934
• UniProt ID: O08796