| Cat.No. : |
FBL-83H |
| Product Overview : |
Recombinant Human Fibrillarin Protein (1-321) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. |
| Description : |
Human recombinant fibrillarin (residues 2-321; Genbank Accession # NM_001436; MW= 61.3 kDa) expressed as an N-terminal GST-fusion protein in E. coli. Fibrillarin is a component of nucleolar ribonucleoprotein complexes including snoRNPs (small nucleolar ribonucleoproteins) and Cajal bodies. As part of the C/D snoRNPs, fibrillarin functions as the catalytic subunit in the 2’-O methylation of ribosomal RNAs1. In addition, fibrillarin has recently been shown to catalyze methylation of histone H2A glutamine-104, a modification restricted to the nucleolus and linked to RNA Pol I transcriptional activity on rDNA3. A distinct protein subcomplex comprising fibrillarin, SF2A-p32 and the argininine methyltransferases PRMT1 and PRMT5 has been described4 and fibrillarin’s N-terminal glycine and arginine-rich (GAR) domain, including in the context of full-length fibrillarin5, is a substrate for various PRMTs. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10 % glycerol |
| Molecular Mass : |
60.3 kDa |
| Protein length : |
1-321 |
| Purity : |
>95% by SDS-PAGE |
| Notes : |
This product is NOT intended for therapeutic or diagnostic use in animals or in humans. |
| Storage : |
Store at -70 centigrade. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended. |
| Figure : |
Coomassie bluestained SDS-PAGE (4-12% acrylamide) of 5 µg of Fibrillarin (GST). MW markers (left) are, from top, 220, 160, 120, 100, 90, 80, 70, 60, 50, 40, 30, 25, 20, 15, & 10 kDa.
Methyltransferase Activity of PRMT5/MEP50 Complex and PRMT5 (alone) with Fibrillarin (GST). Methylation determined as TCA-precipitable
counts in a scintillation/filter plate assay. Reactions were 25 µL, 60 min., 30 centigrade, with 1 µM [3H]-SAM and 0.05 mg/mL Fibrillarin (GST).
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