Recombinant Human GAD2 Protein, GST-tagged

• Cat.No.: GAD2-4655H
• Product Overview: Human GAD2 full-length ORF ( NP_000809.1, 1 a.a. - 585 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 91.8 kDa
• AA Sequence: MASPGSGFWSFGSEDGSGDSENPGT ARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACAC DQKPCSCSKVDVNYAFLHATDLLPA CDGERPTLAFLQDVMNILLQYVVKS FDRSTKVIDFHYPNELLQEYNWELA DQPQNLEEILMHCQTTLKYAIKTGH PRYFNQLSTGLDMVGLAADWLTSTA NTNMFTYEIAPVFVLLEYVTLKKMR EIIGWPGGSGDGIFSPGGAISNMYA MMIARFKMFPEVKEKGMAALPRLIA FTSEHSHFSLKKGAAALGIGTDSVI LIKCDERGKMIPSDLERRILEAKQK GFVPFLVSATAGTTVYGAFDPLLAV ADICKKYKIWMHVDAAWGGGLLMSR KHKWKLSGVERANSVTWNPHKMMGV PLQCSALLVREEGLMQNCNQMHASY LFQQDKHYDLSYDTGDKALQCGRHV DVFKLWLMWRAKGTTGFEAHVDKCL ELAEYLYNIIKNREGYEMVFDGKPQ HTNVCFWYIPPSLRTLEDNEERMSR LSKVAPVIKARMMEYGTTMVSYQPL GDKVNFFRMVISNPAATHQDIDFLI EEIERLGQDL
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: GAD2 glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) [ Homo sapiens ]
• Official Symbol: GAD2
• Synonyms: GAD2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); glutamate decarboxylase 2; GAD65; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; MGC161605; MGC161607
• Gene ID: 2572
• mRNA Refseq: NM_000818
• Protein Refseq: NP_000809
• MIM: 138275
• UniProt ID: Q05329

Reviews

04/14/2023

The GAD2 protein exhibits remarkable performance in Western blotting (WB) experiments.

12/13/2022

The versatility and efficacy of GAD2 in these applications make it an invaluable tool for researchers.

11/11/2019

Researchers can rely on GAD2 to deliver reliable and accurate results in ELISA experiments, ensuring precise measurement and quantification of target proteins.

Q&As

Is there a genetic component related to GAD2 protein and neurological disorders?

Yes, genetic variations in the GAD2 gene have been associated with an increased risk of developing certain neurological disorders, indicating a genetic component in their etiology.

Can GAD2 protein levels be manipulated pharmacologically?

Pharmacological agents targeting GAD2 activity are being explored to modulate GABAergic neurotransmission, potentially offering novel treatments for neurological disorders.

Can GAD2 protein be used as a diagnostic marker for neurological disorders?

Yes, elevated or reduced levels of GAD2 protein can serve as a diagnostic marker for certain neurological disorders, aiding in their identification and management.

How is GAD2 protein linked to neurological disorders?

GAD2 protein is linked to neurological disorders like epilepsy, Parkinson's disease, and various psychiatric conditions due to its role in regulating GABA levels, which are crucial for proper neuronal function.

Are there any therapeutic interventions targeting GAD2 protein?

Research is ongoing to develop therapeutic interventions that modulate GAD2 activity, aiming to restore GABAergic balance in the brain and potentially treat related neurological disorders.