Recombinant Mouse GAD2 cell lysate

Cat.No. : GAD2-001MCL
Product Overview : Mouse GAD65 / GAD2 / GAD-2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
  • Download
Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Tag : Non
Gene Name : Gad2 glutamic acid decarboxylase 2 [ Mus musculus ]
Official Symbol : GAD2
Synonyms : GAD2; glutamic acid decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; glutamate decarboxylase 65 kDa isoform; GAD65; Gad-2; GAD(65); 6330404F12Rik;
Gene ID : 14417
mRNA Refseq : NM_008078
Protein Refseq : NP_032104
Pathway : Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; GABA (gamma-Aminobutyrate) shunt, organism-specific biosystem;
Function : carboxy-lyase activity; catalytic activity; glutamate decarboxylase activity; lyase activity; pyridoxal phosphate binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Reviews
  • Q&As

Customer Reviews (3)

Write a review
Reviews
04/14/2023

    The GAD2 protein exhibits remarkable performance in Western blotting (WB) experiments.

    12/13/2022

      The versatility and efficacy of GAD2 in these applications make it an invaluable tool for researchers.

      11/11/2019

        Researchers can rely on GAD2 to deliver reliable and accurate results in ELISA experiments, ensuring precise measurement and quantification of target proteins.

        Q&As (5)

        Ask a question
        Is there a genetic component related to GAD2 protein and neurological disorders? 07/17/2022

        Yes, genetic variations in the GAD2 gene have been associated with an increased risk of developing certain neurological disorders, indicating a genetic component in their etiology.

        Can GAD2 protein levels be manipulated pharmacologically? 10/08/2020

        Pharmacological agents targeting GAD2 activity are being explored to modulate GABAergic neurotransmission, potentially offering novel treatments for neurological disorders.

        Can GAD2 protein be used as a diagnostic marker for neurological disorders? 12/16/2019

        Yes, elevated or reduced levels of GAD2 protein can serve as a diagnostic marker for certain neurological disorders, aiding in their identification and management.

        How is GAD2 protein linked to neurological disorders? 06/19/2019

        GAD2 protein is linked to neurological disorders like epilepsy, Parkinson's disease, and various psychiatric conditions due to its role in regulating GABA levels, which are crucial for proper neuronal function.

        Are there any therapeutic interventions targeting GAD2 protein? 04/24/2019

        Research is ongoing to develop therapeutic interventions that modulate GAD2 activity, aiming to restore GABAergic balance in the brain and potentially treat related neurological disorders.

        Ask a Question for All GAD2 Products

        Required fields are marked with *

        My Review for All GAD2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends