Recombinant Mouse GAD2 cell lysate
Cat.No. : | GAD2-001MCL |
Product Overview : | Mouse GAD65 / GAD2 / GAD-2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
- Download
Source : | Baculovirus-Insect Cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | Gad2 glutamic acid decarboxylase 2 [ Mus musculus ] |
Official Symbol : | GAD2 |
Synonyms : | GAD2; glutamic acid decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; glutamate decarboxylase 65 kDa isoform; GAD65; Gad-2; GAD(65); 6330404F12Rik; |
Gene ID : | 14417 |
mRNA Refseq : | NM_008078 |
Protein Refseq : | NP_032104 |
Pathway : | Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; GABA (gamma-Aminobutyrate) shunt, organism-specific biosystem; |
Function : | carboxy-lyase activity; catalytic activity; glutamate decarboxylase activity; lyase activity; pyridoxal phosphate binding; |
Products Types
◆ Recombinant Protein | ||
GAD2-956H | Recombinant Human GAD2 Protein, His (Fc)-Avi-tagged | +Inquiry |
GAD2-512M | Recombinant Mouse GAD2 Protein (1-585 aa), His-tagged | +Inquiry |
GAD2-1556H | Recombinant Human GAD2 Protein, His-tagged | +Inquiry |
GAD2-1303M | Recombinant Mouse GAD2 protein(Met1-Leu585) | +Inquiry |
GAD2-1618R | Recombinant Rhesus Macaque GAD2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Reviews
- Q&As
Customer Reviews (3)
Write a reviewThe GAD2 protein exhibits remarkable performance in Western blotting (WB) experiments.
The versatility and efficacy of GAD2 in these applications make it an invaluable tool for researchers.
Researchers can rely on GAD2 to deliver reliable and accurate results in ELISA experiments, ensuring precise measurement and quantification of target proteins.
Q&As (5)
Ask a questionYes, genetic variations in the GAD2 gene have been associated with an increased risk of developing certain neurological disorders, indicating a genetic component in their etiology.
Pharmacological agents targeting GAD2 activity are being explored to modulate GABAergic neurotransmission, potentially offering novel treatments for neurological disorders.
Yes, elevated or reduced levels of GAD2 protein can serve as a diagnostic marker for certain neurological disorders, aiding in their identification and management.
GAD2 protein is linked to neurological disorders like epilepsy, Parkinson's disease, and various psychiatric conditions due to its role in regulating GABA levels, which are crucial for proper neuronal function.
Research is ongoing to develop therapeutic interventions that modulate GAD2 activity, aiming to restore GABAergic balance in the brain and potentially treat related neurological disorders.
Ask a Question for All GAD2 Products
Required fields are marked with *
My Review for All GAD2 Products
Required fields are marked with *
Inquiry Basket