Recombinant Human GCSH Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GCSH-6266H
• Product Overview: GCSH MS Standard C13 and N15-labeled recombinant protein (NP_004474) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 18.91 kDa
• AA Sequence: MALRVVRSVRALLCTLRAVPLPAAP CPPRPWQLGVGAVRTLRTGPALLSV RKFTEKHEWVTTENGIGTVGISNFA QEALGDVVYCSLPEVGTKLNKQDEF GALESVKAASELYSPLSGEVTEINE ALAENPGLVNKSCYEDGWLIKMTLS NPSELDELMSEEAYEKYIKSIEETR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GCSH glycine cleavage system protein H [ Homo sapiens (human) ]
• Official Symbol: GCSH
• Synonyms: GCSH; glycine cleavage system protein H (aminomethyl carrier); glycine cleavage system H protein, mitochondrial; lipoic acid containing protein; lipoic acid-containing protein; mitochondrial glycine cleavage system H-protein; GCE; NKH
• Gene ID: 2653
• mRNA Refseq: NM_004483
• Protein Refseq: NP_004474
• MIM: 238330
• UniProt ID: P23434