Active Recombinant Human GDF15 protein
Cat.No. : | GDF15-246H |
Product Overview : | Recombinant Human GDF15(Ala197-Ile308) was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), Placental Transforming Growth Factor beta, Prostate-derived Factor, and Placental Bone Morphogenetic Protein, is a divergent member of the TGF-beta superfamily. Human GDF-15 shares 66% and 68% amino acid sequence identity with the rat and mouse proteins, respectively. GDF-15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, the GDF-15 proprotein is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. The C-terminal domain of GDF-15 contains seven characteristic conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the mature protein and signals through the heterodimeric receptor composed of TGF-beta RI/ALK-5 and TGF-beta RII. GDF-15 has been shown to have various functions, including inhibition of TNF-alpha production from lipopolysaccharide-stimulated macrophages and the induction of cartilage formation. GDF-15 also promotes neuronal survival, and hypothalamic expression of GDF-15 causes appetite suppression via modulation of Neuropeptide Y and Pro-opiomelanocortin levels. GDF-15 is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, inhibiting CXCL1-induced neutrophil adhesion, regulating angiogenesis, and inhibiting norepinephrine-induced mycardial hypertrophy. |
Source : | E. coli |
Species : | Human |
Predicted N Terminal : | Ala197 |
Form : | Lyophilized from a 0.2 μm filtered solution in HCl. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Human GDF-15 is used at 0.5 µg/mL, the concentration of Recombinant Human Activin RIB/ALK-4 Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-3 μg/mL. |
Molecular Mass : | Predicted Molecular Mass: 12 kDa SDS-PAGE: 12 kDa, reducing conditions |
Endotoxin : | <0.1 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | GDF15 growth differentiation factor 15 [ Homo sapiens ] |
Official Symbol : | GDF15 |
Synonyms : | GDF15; growth differentiation factor 15; growth/differentiation factor 15; MIC 1; MIC1; NAG 1; PDF; PLAB; prostate differentiation factor; PTGFB; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; MIC-1; NAG-1; GDF-15; |
Gene ID : | 9518 |
mRNA Refseq : | NM_004864 |
Protein Refseq : | NP_004855 |
MIM : | 605312 |
UniProt ID : | Q99988 |
Products Types
◆ Recombinant Protein | ||
GDF15-4821H | Active Recombinant Human GDF15 Protein | +Inquiry |
Gdf15-01M | Active Recombinant Mouse Gdf15 Protein, Fc-tagged | +Inquiry |
GDF15-20H | Recombinant Human GDF15 protein(Ala197-Ile308), His-tagged | +Inquiry |
GDF15-2158R | Recombinant Rat GDF15 Protein, His (Fc)-Avi-tagged | +Inquiry |
GDF15-285H | Recombinant Human GDF15 protein, His-tagged | +Inquiry |
◆ Native Protein | ||
GDF15-27680TH | Active Native Human GDF15 Protein | +Inquiry |
◆ Lysates | ||
GDF15-2705HCL | Recombinant Human GDF15 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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