| Species : |
Cynomolgus |
| Source : |
N-His |
| Protein Length : |
Arg193-Val308 |
| Tag : |
N-His |
| Form : |
Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Cynomolgus GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. |
| Molecular Mass : |
The protein has a predicted MW of 14.15 kDa. The protein migrates to 15-18 kDa based on Tris-Bis PAGE result. |
| Endotoxin : |
Less than 1EU per μg by the LAL method. |
| Purity : |
> 95% as determined by Tris-Bis PAGE. |
| Storage : |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Reconstitution : |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
