Recombinant Human Glia Maturation Factor beta

• Cat.No.: GMFB-04H
• Product Overview: Recombinant Human Glia Maturation Factor beta produced in E.coli is a single non-glycosylated polypeptide chain containing 141 amino acids and having a molecular mass of approximately 16.5KDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: GMF-β, a brain-specific protein that belongs to the actin-binding proteins (ADF) structural family. GMF-β appears to play a role in the differentiation, maintenance, and regeneration of the nervous system. It also supports the progression of certain auto-immune diseases, possibly through its ability to induce the production and secretion of various pro-inflammatory cytokines.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Formulation: Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 130mM NaCl.
• Biological Activity: The biological activity was determined by the does-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <0.5ng/ml.
• Endotoxin: Less than 1EU/μg of rHuGMF-β as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: GMFB
• Synonyms: GMF, GMF-beta, Glia maturation factor beta; glia maturation factor, beta; GMFB
• Gene ID: 2764
• mRNA Refseq: NM_004124.2
• Protein Refseq: NP_004115.1
• MIM: 601713
• UniProt ID: P60983
• Chromosome Location: 14q22.2
• Function: actin binding, enzyme activator activity, growth factor activity, protein kinase inhibitor activity, signal transducer activity.