Recombinant Human Glycerol-3-phosphate dehydrogenase 1 (soluble)

• Cat.No.: GPD1-1942H
• Product Overview: Recombinant human GPD1 protein was expressed inE.coliand purified by using conventional chromatography techniques. 37.5 kDa (349aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: GPD1, also known as glycerol-3-phosphate dehydrogenase, is an enzyme that catalyzes the reduction of dihydroxyacetone phosphate to sn-glycerol 3-phosphate. (sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH) Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase and glycerolphosphate dehydrogenase. However, GPD1 is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol.
• Purity: > 90% by SDS – PAGE.
• Physical Appearance: Liquid.
• Formulation: 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILIFVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKELMQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRNRKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Glycerophospholipid metabolism; Metabolism of lipids and lipoproteins

Gene Information

• Gene Name: GPD1 glycerol-3-phosphate dehydrogenase 1 (soluble) [ Homo sapiens ]
• Synonyms: GPD1; glycerol-3-phosphate dehydrogenase 1 (soluble); FLJ26652; glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; GPD-C; GPDH-C; glycerol-3-phosphate dehydrogenase, soluble; EC 1.1.1.8
• Gene ID: 2819
• mRNA Refseq: NM_005276
• Protein Refseq: NP_005267
• MIM: 138420
• UniProt ID: P21695
• Chromosome Location: 12q12-q13
• Function: NAD or NADH binding; binding; glycerol-3-phosphate dehydrogenase (NAD+) activity; glycerol-3-phosphate dehydrogenase activity; protein homodimerization activity

Citation

Temporal activation of WNT/β-catenin signaling is sufficient to inhibit SOX10 expression and block melanoma growth

Journal: Oncogene    PubMed ID: 32238882    Data: 2020/4/1

Authors: Rexhep Uka, Christian Britschgi, Olga Shakhova

Article Snippet:To identify novel SOX10-interacting proteins, a M010817 WCE was analyzed by nLc Orbitrap MS at CovalX (CovalX AG, Zürich, Switzerland).To identify novel SOX10-interacting proteins, a M010817 WCE was analyzed by nLc Orbitrap MS at CovalX (CovalX AG, Zürich, Switzerland).. M010817 were lysed in NE buffer (50 mM Tris pH 7.5, 150 mM KCl, 0.2 mM EDTA, 5 mM MgCl 2 , 20% Glycerol, 0.5 mM DTT) and then sent to CovalX for further analysis, along with purified SOX10 protein in a buffer of 25 mM Tris pH 7.3, 100 mM glycine, 10% glycerol, obtained from Creative BioMart (Creative BioMart Inc, Shirley, NY, USA). . Procedure at CovalX were as follows:Procedure at CovalX were as follows: