Recombinant Human GPI Protein, His-tagged GPI-009H

Recombinant Human GPI Protein, His-tagged

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Recombinant Human GPI Protein, His-tagged

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Cat.No. : GPI-009H
Product Overview : Recombinant Human GPI protein (2-558) with a C-terminal His-tag was expressed in E. coli
Description : This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 66.0 kDa
Protein length : 2-558
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to reduce 1 µmol of NADP + to NADPH per minute at 25 centigrade in 50 mM Triethanolamine, pH 7.6, containing 1 mM MgCl2.
Stability : ≥ 2 years
Storage : -80 centigrade
Storage Buffer : 25 mM Tris-HCl, pH 7.0, 100 mM sodium chloride, and 10% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.
SDS-PAGE Analysis of GPI :

Lane 1: MW Markers
Lane 2: GPI (human recombinant) (1 µg)
Lane 3: GPI (human recombinant) (2 µg)

Gene Name : GPI glucose-6-phosphate isomerase [ Homo sapiens (human) ]
Official Symbol : GPI
Synonyms : GPI; glucose-6-phosphate isomerase; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; glucose-6-phosphate isomerase; autocrine motility factor; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; oxoisomerase; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; sperm antigen-36; EC 5.3.1.9
Gene ID : 2821
mRNA Refseq : NM_000175
Protein Refseq : NP_000166
MIM : 172400
UniProt ID : P06744

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