Active Recombinant Human GRK7 Protein, GST-tagged

• Cat.No.: GRK7-5360H
• Product Overview: Human GRK7 (NP_631948.1, 1 a.a. - 553 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. [provided by RefSeq
• Source: Insect Cell
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: Modified PLKtide.
  ATP: 100 μM.
• Molecular Mass: 89 kDa
• Purity: 97 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: GRK7 G protein-coupled receptor kinase 7 [ Homo sapiens ]
• Official Symbol: GRK7
• Synonyms: GRK7; G protein-coupled receptor kinase 7; GPRK7; g protein-coupled receptor kinase GRK7
• Gene ID: 131890
• mRNA Refseq: NM_139209
• Protein Refseq: NP_631948
• MIM: 606987
• UniProt ID: Q8WTQ7