Recombinant Human HDAC1 293 Cell Lysate

Cat.No. : HDAC1-5608HCL
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Description : Antigen standard for histone deacetylase 1 (HDAC1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : HDAC1 histone deacetylase 1 [ Homo sapiens ]
Official Symbol : HDAC1
Synonyms : HDAC1; histone deacetylase 1; RPD3L1; GON 10; HD1; reduced potassium dependency, yeast homolog-like 1; RPD3; GON-10; DKFZp686H12203;
Gene ID : 3065
mRNA Refseq : NM_004964
Protein Refseq : NP_004955
MIM : 601241
UniProt ID : Q13547
Chromosome Location : 1p34
Pathway : Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem;
Function : NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); RNA polymerase II transcription corepressor activity; activating transcription factor binding; core promoter binding; enzyme binding; histone deacetylase activity; histone deacetylase activity; histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; hydrolase activity; protein binding; protein deacetylase activity; protein deacetylase activity; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription factor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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Customer Reviews (3)

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    The HDAC1 proteins purchased from your company is suitable for ELISA.


      The shipping was prompt enough for me to get into the experiment in the shortest time possible.


        Friendly customer service and detailed answers to all the questions I asked!

        Q&As (4)

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        What diseases has HDAC1 been associated with the study of? 08/13/2022

        HDAC1 is closely related to the study of diseases such as lung cancer and esophageal squamous carcinoma.

        What is the relationship between HDAC1 and Adriamycin? 07/14/2022

        Adriamycin specifically reduces HDAC1 protein expression.

        How is HDAC1 expressed in esophageal squamous carcinoma tissues? 01/25/2022

        HDAC1 protein is highly expressed in esophageal squamous carcinoma tissues, and its high expression correlates with the development of esophageal squamous carcinoma.

        What biological function does HDAC1 have? 03/13/2021

        HDAC1 is a key enzyme in the histone deacetylation process and is involved in the regulation of histone acetylation levels.

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