Species : |
Human |
Source : |
Mammalian Cells |
Tag : |
DDK&Myc |
Protein Length : |
Met1-Ser519 |
Description : |
Ikaros (also LyF1) is a 60 kDa member of the C2H2-type zinc-finger protein family. It is found in both T and B cells and serves as a context‑dependent activator or repressor of genes. Human Ikaros is 519 amino acids (aa) in length. It possesses two zinc‑finger domains, one at the N‑terminus that contains four zinc-finger motifs (aa 117‑224) and one at the C‑terminus that contains two zinc‑finger motifs (aa 462‑514) . The C‑terminal motifs mediate homo- or heterodimerization, while the N‑terminal motifs bind DNA. Multiple splice forms of 37 kDa to 47 kDa exist (Ikaros 2‑8) with reduced numbers of N‑terminal zinc-finger motifs. At least three are needed for DNA binding, and a heterodimer with a two‑motif monomer is suggested to be transcriptionally inert. Over aa 427‑519 (which are not spliced), human Ikaros shows 88% aa identity to mouse Ikaros. |
Form : |
Lyophilized powder/frozen liquid |
Molecular Mass : |
57.09 kDa |
Endotoxin : |
< 1.0 EU/μg of the protein as determined by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Notes : |
For research use only. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt. |
Storage Buffer : |
Supplied as solution form in PBS or lyophilized from PBS. |
Reconstitution : |
Reconstitute in sterile water for a stock solution. |
Shipping : |
They are shipped out with dry ice/blue ice unless customers require otherwise. |