Recombinant Human IKZF1 Protein (Met1-Ser519), C-MYC/DDK tagged

• Cat.No.: IKZF1-3889H
• Product Overview: Recombinant human IKZF1 (Met1-Ser519) fused with the C-terminal MYC/DDK tag was expressed in Mammalian cells.

Specification

• Description: Ikaros (also LyF1) is a 60 kDa member of the C2H2-type zinc-finger protein family. It is found in both T and B cells and serves as a context‑dependent activator or repressor of genes. Human Ikaros is 519 amino acids (aa) in length. It possesses two zinc‑finger domains, one at the N‑terminus that contains four zinc-finger motifs (aa 117‑224) and one at the C‑terminus that contains two zinc‑finger motifs (aa 462‑514) . The C‑terminal motifs mediate homo- or heterodimerization, while the N‑terminal motifs bind DNA. Multiple splice forms of 37 kDa to 47 kDa exist (Ikaros 2‑8) with reduced numbers of N‑terminal zinc-finger motifs. At least three are needed for DNA binding, and a heterodimer with a two‑motif monomer is suggested to be transcriptionally inert. Over aa 427‑519 (which are not spliced), human Ikaros shows 88% aa identity to mouse Ikaros.
• Source: Mammalian cells
• Species: Human
• Tag: C-MYC/DDK
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 57.09 kDa
• Protein Length: Met1-Ser519
• Endotoxin: < 1.0 EU/μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS or lyophilized from PBS.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: IKZF1 IKAROS family zinc finger 1 (Ikaros) [ Homo sapiens (human) ]
• Official Symbol: IKZF1
• Synonyms: IKZF1; IKAROS family zinc finger 1 (Ikaros); zinc finger protein, subfamily 1A, 1 (Ikaros), ZNFN1A1; DNA-binding protein Ikaros; hIk 1; Hs.54452; IKAROS; LyF 1; CLL-associated antigen KW-6; Ikaros (zinc finger protein); ikaros family zinc finger protein 1; lymphoid transcription factor LyF-1; zinc finger protein, subfamily 1A, 1 (Ikaros); IK1; LYF1; hIk-1; PRO0758; ZNFN1A1
• Gene ID: 10320
• mRNA Refseq: NM_001220765
• Protein Refseq: NP_001207694
• MIM: 603023
• UniProt ID: Q13422