Recombinant Human IL10 protein

Cat.No. : IL10-16H
Product Overview : Recombinant Human IL10 protein was expressed in Escherichia coli.
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Description : Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10Rβ does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
Molecular Mass : Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
Protein length : 160
AA Sequence : SPGQGTQSENSCTHFPGNLPNMLRD LRDAFSRVKTFFQMKDQLDNLLLKE SLLEDFKGYLGCQALSEMIQFYLEE VMPQAENQDPDIKAHVNSLGENLKT LRLRLRRCHRFLPCENKSKAVEQVK NAFNKLQEKGIYKAMSEFDIFINYI EAYMTMKIRN
Endotoxin : Less than 1 EU/µg of rHuIL-10 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Publication :
Relationship between IL-10 and PD-L1 in esophageal carcinoma tissues and IL-10 down-regulates PD-L1 expression via Met signaling pathway (2020)
SDS-PAGE:
Gene Name : IL10
Official Symbol : IL10
Synonyms : IL10; interleukin 10; interleukin-10; CSIF; cytokine synthesis inhibitory factor; IL 10; IL10A; T cell growth inhibitory factor; TGIF; T-cell growth inhibitory factor; GVHDS; IL-10; MGC126450; MGC126451;
Gene ID : 3586
mRNA Refseq : NM_000572
Protein Refseq : NP_000563
MIM : 124092
UniProt ID : P22301

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What state-of-the-art experimental strategies, including advanced imaging techniques, are implemented to meticulously assess the in vivo efficacy of IL-10-based therapies across a spectrum of inflammatory disorders? 02/12/2022

Cutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.

How are advanced methods like single-cell RNA sequencing and flow cytometry harnessed to investigate the immunomodulatory impact of IL-10 across distinct immune cell subtypes? 02/10/2022

Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.

Could you elaborate on the intricate experimental methodologies applied to unravel the influence of genetic polymorphisms within IL-10 and its receptor on disease susceptibility? 10/26/2021

Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.

How do researchers leverage advanced immunomonitoring tools to comprehensively evaluate the safety profiles of IL-10-based interventions in various clinical trial phases? 05/25/2021

Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.

How do researchers employ in vitro models to elucidate the mechanisms underlying IL-10's anti-inflammatory effects on immune cells? 12/03/2020

In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.

What sophisticated experimental techniques are utilized to dissect the intricate signaling pathways downstream of IL-10 activation? 08/26/2020

State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.

Could you explain the multifaceted experimental strategies employed to determine the optimal dosage and administration routes for IL-10 in diverse animal models? 03/24/2019

Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.

What innovative experimental designs are employed to explore potential synergies between IL-10 and other cytokines, unraveling complex interplay in immune regulation? 11/23/2018

Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.

Can you elaborate on the comprehensive approaches scientists use to assess the tissue-specific expression patterns of IL-10 receptors in both normal and pathological conditions? 11/05/2018

Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.

How are cutting-edge techniques such as CRISPR/Cas9 gene editing utilized to dissect the regulatory roles of IL-10 in complex autoimmune disease models? 02/22/2018

CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.

Customer Reviews (3)

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Reviews
09/27/2022

    Effective in anti-inflammatory assays.

    01/09/2019

      High purity levels.

      04/15/2017

        Stable under long-term storage.

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