Recombinant Human Interleukin 10

• Cat.No.: IL10-110H
• Product Overview: Recombinant Human interleukin 10 encoding the human IL-10 protein sequence (containing the signal peptide sequence, and the mature human Interleukin 10 sequence) was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: IL10-110H
• Description: Interleukin 10 (IL-10) is a pleiotropic cytokine that regulates multiple immune responses through actions on T cells, B cells, macrophage/monocytes and antigen presenting cells (APC) and generally skews the immune response from TH1 to TH2. IL-10 may suppress immune responses by inhibiting expression of IL-1a, IL-1b, IL-6, IL-8, TNF-a, GM-CSF and G-CSF in activated monocytes and activated macrophages. IL-10 also suppresses IFN-g production by NK cells.
• Source: Human 293 cells.
• Amino Acid Sequence: SPGQGTQ SEN SCTHFP G NLPNMLRDL RDA FSRV KTFFQMK DQLDNLLLK ESLLEDFK GY LGC QAL SEMIQ FYLE EVMPQA ENQ DPDIKA HVNSLGENL KTLRL RLRRCH RFL PC E NK SKAVEQVK NAFN KLQEKGIYKAM SEFDIFI NYIEA YMTMKIRN.
• Molecular Mass: Interleukin 10 migrates as a band between 10 and 20 kDa in SDS-PAGE due. This compares with predicted molecular mass of 18.6 kDa.
• pI: Interleukin 10 separates into a number of isoforms with a pI between 3.0 and 9.0 in 2D PAGE due to post-translational modifications. This compares with the unmodified IL-10 that has a predicted pI of 7.63.
• Purity: >95%, as determined by SDS-PAGE and visualized by Silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Gene Information

• Gene Name: IL10 interleukin 10 [ Homo sapiens ]
• Synonyms: IL10; interleukin 10; CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451; Interleukin-10; Cytokine synthesis inhibitory factor; MGC126450; MGC126451
• Gene ID: 3586
• mRNA Refseq: NM_000572
• Protein Refseq: NP_000563
• MIM: 124092
• UniProt ID: P22301
• Chromosome Location: 1q31-q32
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-10 receptor binding ; protein binding