Recombinant Human IL12B

• Cat.No.: IL12B-220H
• Product Overview: Recombinant Human interleukin 12B DNA sequences encoding the p40 subunit of the human IL-12 protein was expressed inSf 21 insect cells.

Specification

• Cat. No.: IL12B-220H
• Description: Interleukin 12 (IL-12, NK cell stimulatory factor, cytotoxic lymphocyte maturation factor) is a heterodimeric cytokine that is naturally produced by dendritic cells , macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity, which can block the formation of new blood vessels. IL-12 binds to the IL-12 receptor and upon binding, IL-12R-β2 becomes tyrosine phosphorylated and provides binding sites for kinases, Tyk2 and Jak2. These are important in activating critical transcription factor proteins such as STAT4 which are implicated in IL-12 signaling in T cells and NK cells.
• Source: Sf21 Insect Cells.
• Molecular Weight: The predicted molecular weight of Recombinant Human IL-12 is Mr 40 kDa.
• State Of Matter: Lyophilized.
• Formulation: This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 μg of bovine serum albumin per 1 μg of cytokine with no calcium, magnesium, or preservatives.
• Purity: > 90% by SDS Page and analyzed by silver stain.
• Endotoxin: < 1.0 EU/µg as determined by the LAL method.
• Biological Activity: The monomeric human IL-12 p40 monomer exhibits no IL-12 antagonist activity. rhIL-12 p40 is intended for use as a control for anti-human IL-12 p40 antibodies.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
• Tag: Non

Gene Information

• Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Synonyms: OTTHUMP00000160820; CLMF; CLMF2; IL-12B; NKSF; NKSF2; CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; IL12, subunit p40; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); IL12B
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• UniProt ID: P29460
• Chromosome Location: 5q31.1-q33.1
• MIM: 161561
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus
• Function: contributes_to cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-12 alpha subunit binding; interleukin-12 receptor binding; protein binding; protein homodimerization activity; signal transducer activity

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.