Recombinant Sheep IL12B Protein (23-327 aa), His-tagged

• Cat.No.: IL12B-576S
• Product Overview: Recombinant Sheep IL12B Protein (23-327 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates mory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
• Source: E. coli
• Species: Sheep
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 38.5 kDa
• Protein length: 23-327 aa
• AA Sequence: IWELEKNVYVVELDWYPNAPGETVV LTCDTPEEDGITWTSDQSSEVLGSG KTLTIQVKEFGDAGQYTCHKGGEVL SRSLLLLHKKEDGIWSTDILKDQKE PKAKSFLKCEAKDYSGHFTCSWLTA ISTNLKFSVKSSRGSSDPRGVTCGA ASLSAEKVSMDHREYNKYTVECQEG SACPAAEESLPIEVVMEAVHKLKYE NYTSSFFIRDIIKPDPPKNLQLRPL KNSRQVEVSWEYPDTWSTPHSYFSL TFCVQVQGKNKREKKLFTDQTSAKV TCHKDANIRVQARDRYYSSFWSEWA SVSCS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Ovis aries ]
• Official Symbol: IL12B
• Synonyms: IL12B; IL-12B; CLMF p40; IL-12 subunit p40; IL-12
• Gene ID: 443472
• mRNA Refseq: NM_001009438
• Protein Refseq: NP_001009438
• UniProt ID: P68220

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.