Recombinant Sheep IL12B Protein (23-327 aa), His-tagged

Cat.No. : IL12B-576S
Product Overview : Recombinant Sheep IL12B Protein (23-327 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.
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Description : Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates mory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Source : E. coli
Species : Sheep
Tag : His
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 38.5 kDa
Protein length : 23-327 aa
AA Sequence : IWELEKNVYVVELDWYPNAPGETVV LTCDTPEEDGITWTSDQSSEVLGSG KTLTIQVKEFGDAGQYTCHKGGEVL SRSLLLLHKKEDGIWSTDILKDQKE PKAKSFLKCEAKDYSGHFTCSWLTA ISTNLKFSVKSSRGSSDPRGVTCGA ASLSAEKVSMDHREYNKYTVECQEG SACPAAEESLPIEVVMEAVHKLKYE NYTSSFFIRDIIKPDPPKNLQLRPL KNSRQVEVSWEYPDTWSTPHSYFSL TFCVQVQGKNKREKKLFTDQTSAKV TCHKDANIRVQARDRYYSSFWSEWA SVSCS
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
Gene Name : IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Ovis aries ]
Official Symbol : IL12B
Synonyms : IL12B; IL-12B; CLMF p40; IL-12 subunit p40; IL-12;
Gene ID : 443472
mRNA Refseq : NM_001009438
Protein Refseq : NP_001009438
UniProt ID : P68220

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
04/08/2020

    Good for cytokine assays.

    01/06/2020

      Effective in Th2 cell differentiation.

      01/12/2019

        High yield in expression.

        Q&As (10)

        Ask a question
        Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation? 05/12/2021

        ChIP-seq identifies regulatory elements controlling IL12B gene expression.

        Can you elaborate on the use of reporter assays to monitor IL12B promoter activity? 05/02/2021

        Reporter assays monitor IL12B promoter activity under varying conditions.

        What methods evaluate IL12B's response to different pathogenic stimuli in primary cells? 10/30/2020

        Primary cell stimulation assays gauge IL12B response to pathogens.

        How does siRNA knockdown of IL12B elucidate its role in immune cell activation? 05/25/2020

        siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

        How does recombinant protein expression and purification aid in IL12B functional studies? 04/30/2020

        Recombinant protein expression enables functional IL12B studies in vitro.

        How does co-immunoprecipitation validate IL12B's association with signaling partners? 04/12/2018

        Co-IP validates IL12B's binding to specific signaling partners.

        What techniques are employed to analyze the impact of IL12B genetic variants on protein function? 12/03/2017

        Genetic variant analysis reveals impacts on IL12B protein function.

        How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets? 10/31/2017

        Flow cytometry profiles IL12B expression across diverse immune cell types.

        What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation? 09/29/2017

        Cytokine secretion assays measure IL12B's response kinetics to stimuli.

        What are the methods to assess IL12B's protein-protein interactions in a cellular context? 08/22/2017

        Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.

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