Active Recombinant Human IL13 protein, hFc-tagged
Cat.No. : | IL13-147H |
Product Overview : | Recombinant Human IL13 protein(AAK53823.1)(Gly21-Asn132), fused with hFc tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | hFc |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL13RA2 at 8 μg/ml (100 μl/well) can bind IL13 with a linear range of 0.25-8.0 ng/ml. |
Molecular Mass : | The recombinant human Fc/IL13 chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 349 amino acids and predicts a molecular mass of 39 kDa. The apparent molecular mass of rhIL13/Fc monomer is approximately 50-55 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Proteinlength : | Gly21-Asn132 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 98 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | IL13 interleukin 13 [ Homo sapiens ] |
Official Symbol : | IL13 |
Synonyms : | IL13; interleukin 13; interleukin-13; allergic rhinitis; ALRH; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; MGC116786; MGC116788; MGC116789; P600; Bronchial hyperresponsiveness-1 (bronchial asthma); IL-13; |
Gene ID : | 3596 |
mRNA Refseq : | NM_002188 |
Protein Refseq : | NP_002179 |
MIM : | 147683 |
UniProt ID : | P35225 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionFlow cytometry quantifies IL13 receptor expression on cells.
Transgenic models illuminate IL13's in vivo functions.
ELISA quantifies IL13 secretion in cell cultures accurately.
siRNA knockdown uncovers IL13's specific cellular effects.
Co-immunoprecipitation identifies IL13 signaling partners.
Differential techniques distinguish IL13 and IL4 signaling pathways.
Reporter gene assays gauge IL13-driven transcription.
Microarray analysis profiles IL13-induced gene expression.
Experimental methods reveal IL13's impact on tissue remodeling.
Site-directed mutagenesis identifies key residues in IL13 receptor binding.
Customer Reviews (3)
Write a reviewEffective in Th2 cell differentiation.
Good for cytokine assays.
High yield in expression.
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