Recombinant Human IL13 cell lysate

Cat.No. : IL13-2922HCL
Product Overview : Human IL-13 / Interleukin-13 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL13 interleukin 13 [ Homo sapiens ]
Official Symbol : IL13
Synonyms : IL13; interleukin 13; interleukin-13; allergic rhinitis; ALRH; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; MGC116786; MGC116788; MGC116789; P600; Bronchial hyperresponsiveness-1 (bronchial asthma); IL-13;
Gene ID : 3596
mRNA Refseq : NM_002188
Protein Refseq : NP_002179
MIM : 147683
UniProt ID : P35225
Chromosome Location : 5q31
Pathway : Asthma, organism-specific biosystem; Asthma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem; Fc epsilon RI signaling pathway, conserved biosystem;
Function : cytokine activity; interleukin-13 receptor binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does flow cytometry help quantify IL13 receptor expression on different cell populations? 12/29/2022

Flow cytometry quantifies IL13 receptor expression on cells.

What are the benefits of using transgenic animal models to study IL13's in vivo functions? 07/05/2022

Transgenic models illuminate IL13's in vivo functions.

Could you explain the role of ELISA in quantifying IL13 secretion levels in cell cultures? 02/23/2022

ELISA quantifies IL13 secretion in cell cultures accurately.

How can siRNA knockdown experiments reveal IL13's effects on specific cellular processes? 06/13/2021

siRNA knockdown uncovers IL13's specific cellular effects.

How does co-immunoprecipitation identify interacting partners in IL13-mediated signaling complexes? 07/28/2019

Co-immunoprecipitation identifies IL13 signaling partners.

What techniques can differentiate IL13-mediated signaling pathways from IL4 pathways? 07/23/2019

Differential techniques distinguish IL13 and IL4 signaling pathways.

Could you elaborate on the use of reporter gene assays to assess IL13-driven transcriptional activity? 12/16/2018

Reporter gene assays gauge IL13-driven transcription.

Can you describe the applications of microarray analysis in profiling IL13-induced gene expression changes? 11/14/2018

Microarray analysis profiles IL13-induced gene expression.

What experimental methods determine the impact of IL13 on tissue remodeling processes? 08/04/2018

Experimental methods reveal IL13's impact on tissue remodeling.

How does site-directed mutagenesis help identify critical residues in IL13 receptor binding? 07/04/2018

Site-directed mutagenesis identifies key residues in IL13 receptor binding.

Customer Reviews (3)

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Reviews
11/29/2021

    Effective in Th2 cell differentiation.

    04/22/2020

      Good for cytokine assays.

      01/26/2019

        High yield in expression.

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