| Cat. No. : |
IL13-262S |
| Description : |
IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. |
| Source : |
Yeast. |
| Formulation/ Reconstitution : |
Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. |
| Purification : |
Ion-exchange chromatography. |
| Purity : |
>95% as visualized by SDS-PAGE analysis. |
| Amino Acid Sequence : |
LTCFGGLASP GPVPPHSTAL KELIEELVNI TQNQKTPLCN GSMVWSVNLT TSMYCAALES LINISDCSAI QKTQRMLSAL CSHKPPSEQV PGKHIRDTKI EVAQFVKDLL KHLRMIFRHG. |
| Bioactivity : |
The biological activity of recombinant swine IL-13 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 250 - 375 ng/mL. |
| Stability And Storage : |
Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles. |
