|Product Overview:||Recombinant Human IL2 Protein is expressed from E.coli cells. It contains AA Ala 21 - Thr 153 (Accession # P60568). Predicted N-terminus: Ala 21. This protein carries no "tag". The protein has a calculated MW of 15.4 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH5.0. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Human IL-2 R alpha, Fc Tag at 2μg/mL (100 μL/well) can bind Recombinant Human IL2 Protein with a linear range of 0.2-1.56 ng/mL (QC tested).
Human IL-2 R beta, Fc Tag captured on CM5 chip via anti-human IgG Fc antibody, can Recombinant Human IL2 Protein with an affinity constant of 0.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Recombinant Human IL2 Protein stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.284-0.355 ng/ml (Routinely tested).
|Molecular Mass:||15.4 kDa|
|Protein length:||Ala 21 - Thr 153|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||IL2 interleukin 2 [ Homo sapiens ]|
|Synonyms:||IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; IL-2; lymphokine;|