| Cat. No. : |
IL10-217H |
| Description : |
Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is a homodimer protein produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and the Type 1 T helper cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. It is released by cytotoxic T-cells to inhibit the actions of NK cells during the immune response to viral infection. IL-10 plays a key role in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens. |
| Source : |
Sf 21 Insect Cells. |
| Molecular Weight : |
The predicted molecular weight of Recombinant Human IL-10 is Mr 18.6 kDa. |
| State Of Matter : |
Lyophilized. |
| Formulation : |
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present. |
| Purity : |
> 97% by SDS Page and analyzed by silver stain. |
| Endotoxin : |
< 1.0 EU/µg as determined by the LAL method. |
| Biological Activity : |
The biological activity of Human IL-10 was determined by a cell proliferation assay using the murine mast cell line, MC/9-2. The expected ED50 for this effect is typically 0.15 - 0.75 ng/mL. The cell number is assessed in a fluorometric assay using the redox senstitive dye, Resazurin. |
| Storage And Stability : |
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20˚C to -70˚C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2˚C - 8˚C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20˚C to -70˚C in a manual defrost freezer. Avoid repeated freeze thaw cycles. |
