Recombinant Human Janus Kinase 2, GST-His-tagged

• Cat.No.: JAK2-5441H
• Product Overview: Recombinant humanJAK2, containing the catalytic and the pseudo kinase domain (aa 532-1132),with N-terminal GST-His Tag.

Specification

• Cat. No.: JAK2-5441H
• Description: JAK2 is a member ofintracellular non-receptor tyrosine kinases that transduce cytokine-mediatedsignals via the JAK-STAT pathway. JAK2 has two near-identicalphosphate-transferring domains. One domain exhibits the kinase activity whilethe other stabilizes the JAK conformational structure. JAK2 is thepredominant JAK kinase activated in response to several growth factors andcytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to beconstitutively associated with the prolactin receptor and is required forresponses to gamma interferon.
• Source: Sf9 cells
• Species: Human
• Form: Liquid in 25 mMTris-HCl, pH 8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
• Molecular Weight: 99 kda
• Specific Activity: 0.2 U/µg. One unitis defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosinesubstrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4 +3 mM MgCl2 + 3 mM MnCl2 + 1 mM DTT + 3 µM Na-orthovanadate + 0.5 mM ATP + 30µg/ml Poly (Glu:Tyr) 4:1 substrate + 10 µg/ml recombinant JAK2.
• Storage: Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: JAK2

Gene Information

• Gene Name: JAK2 Janus kinase 2 [ Homosapiens ]
• Synonyms: JAK2; Janus kinase 2; JTK10;tyrosine-protein kinase JAK2; Janus kinase 2 (a protein tyrosine kinase); EC2.7.10.2; JAK-2; OTTHUMP00000021024; OTTHUMP00000043260
• Gene ID: 3717
• mRNA Refseq: NM_004972
• Protein Refseq: NP_004963
• MIM: 147796
• UniProt ID: O60674
• Chromosome Location: 9p24
• Pathway: Adipocytokine signaling pathway; CXCR4-mediatedsignaling events; Chemokine signaling pathway; Cholinergic synapse; Delta-NotchSignaling Pathway; EGFR1 Signaling Pathway; EPO Receptor Signaling; ErbB2/ErbB3signaling events; ErbB4 signaling events; Growth hormone receptor signaling; Herpessimplex infection; IFN-gamma pathway; IL-2 Signaling Pathway; Immune System
• Function: ATP binding; Janus kinaseactivity; SH2 domain binding; acetylcholine receptor binding; growth hormonereceptor binding; insulin receptor substrate binding; interleukin-12 receptorbinding; non-membrane spanning protein tyrosine kinase activity; nucleotidebinding; peptide hormone receptor binding; phosphoinositide 3-kinase binding;transferase activity