Recombinant Human Janus Kinase 2, His-tagged

• Cat.No.: JAK2-532H
• Product Overview: Recombinant human JAK2 was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=42.5kDa.

Specification

• Cat. No.: Jak2-532H
• Description: JAK2 is a member of intracellular non-receptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway. JAK2 has two near-identical phosphate-transferring domains. One domain exhibits the kinase activity while the other stabilizes the JAK conformational structure. JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon.
• Source: Sf9 insect cells.
• Purity: >90%.
• Specific Activity: 118 pmol/min/μg, Assay conditions: 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (Tyr 4, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol, and 1 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: JAK2 Janus kinase 2 [ Homo apiens ]
• Synonyms: JAK2; Janus kinase 2; JTK10; tyrosine-protein kinase JAK2; Janus kinase 2 (a protein tyrosine kinase); EC 2.7.10.2; JAK-2; OTTHUMP00000021024; OTTHUMP00000043260
• Gene ID: 3717
• mRNA Refseq: NM_004972
• Protein Refseq: NP_004963
• MIM: 147796
• UniProt ID: O60674
• Chromosome Location: 9p24
• Pathway: Adipocytokine signaling pathway; Chemokine signaling pathway; Jak-STAT signaling pathway
• Function: ATP binding; Janus kinase activity; SH2 domain binding; acetylcholine receptor binding; growth hormone receptor binding; insulin receptor substrate binding; interleukin-12 receptor binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; peptide hormone receptor binding; phosphoinositide 3-kinase binding; transferase activity

Reviews

10/03/2020

Quick response from customer service.

05/12/2020

Product performed as expected.

08/07/2018

Satisfied with product quality.

Q&As

What role does JAK2 play in myeloproliferative neoplasms (MPNs)?

JAK2 mutations, particularly JAK2 V617F, are hallmarks of several MPNs, including polycythemia vera, essential thrombocythemia, and primary myelofibrosis. These mutations lead to uncontrolled cell proliferation.

How does JAK2 activation lead to downstream signal transduction?

Upon cytokine binding to its receptor, JAK2 becomes activated through phosphorylation. This activation leads to the phosphorylation and activation of STAT (Signal Transducers and Activators of Transcription) proteins, which then translocate to the nucleus and regulate gene expression.

What is the primary function of the JAK2 protein in cellular signaling?

JAK2 is a non-receptor tyrosine kinase that plays a pivotal role in cytokine signaling, mediating cellular responses to growth factors and various cytokines.

How is JAK2 involved in hematopoiesis and blood cell development?

JAK2 is crucial for hematopoiesis, especially in the signaling pathways of erythropoietin and thrombopoietin, which are essential for red blood cell and platelet production, respectively.

How does JAK2 interact with cytokine receptors, and what is its significance?

JAK2 associates with the intracellular domains of specific cytokine receptors. Upon cytokine binding, JAK2 becomes activated, leading to downstream signaling events. This interaction is vital for mediating cellular responses to various cytokines.

Are there therapeutic agents that target JAK2, and in what contexts are they used?

Yes, there are several JAK2 inhibitors, such as ruxolitinib and fedratinib, which are used to treat MPNs. They work by inhibiting the kinase activity of JAK2, thereby reducing aberrant cell proliferation.

Are there known mutations in the JAK2 gene, and what are their implications?

Yes, one of the most well-known mutations is JAK2 V617F, which results in constitutive activation of the kinase. This mutation is associated with various myeloproliferative neoplasms (MPNs).