Recombinant Human KDM5A Protein (437-603 aa), His-SUMO-tagged

• Cat.No.: KDM5A-597H
• Product Overview: Recombinant Human KDM5A Protein (437-603 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Transcription. Protein Description: Full Length of Mature Protein.

Specification

• Description: Histone dethylase that specifically dethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not dethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Dethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone dethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 35.3 kDa
• Protein length: 437-603 aa
• AA Sequence: EYALSGWNLNNMPVLEQSVLAHINV DISGMKVPWLYVGMCFSSFCWHIED HWSYSINYLHWGEPKTWYGVPSHAA EQLEEVMRELAPELFESQPDLLHQL VTIMNPNVLMEHGVPVYRTNQCAGE FVVTFPRAYHSGFNQGYNFAEAVNF CTADWLPIGRQCVNHYR
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: KDM5A lysine (K)-specific demethylase 5A [ Homo sapiens ]
• Official Symbol: KDM5A
• Synonyms: KDM5A; RBP2; RBBP2; RBBP-2; JARID1A
• Gene ID: 5927
• mRNA Refseq: NM_001042603
• Protein Refseq: NP_001036068
• MIM: 180202
• UniProt ID: P29375