Recombinant Human LCK protein, Flag-tagged, Biotinylated

• Cat.No.: LCK-60H
• Product Overview: Recombinant Human LCK [1-509(end) amino acids of accession number NP_005347.2] fused with DYKDDDDK tag at N-terminal was expressed in insect cell and biotinylated.

Specification

• Source: Insect cell
• Species: Human
• Tag: Flag
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Molecular Mass: 62 kDa
• Purity: >90% as determined by SDS-PAGE and CBB staining
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: LCK lymphocyte-specific protein tyrosine kinase [ Homo sapiens ]
• Official Symbol: LCK
• Synonyms: LCK; lymphocyte-specific protein tyrosine kinase; LSK; YT16; p56lck; pp58lck
• Gene ID: 3932
• mRNA Refseq: NM_001042771
• Protein Refseq: NP_001036236
• MIM: 153390
• UniProt ID: P06239
• Chromosome Location: 1p34.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Alpha-synuclein signaling, organism-specific biosystem; Atypical NF-kappaB pathway, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem; CD28 dependent Vav1 pathway, organism-specific biosystem
• Function: ATP binding; ATPase binding; CD4 receptor binding; CD8 receptor binding; SH2 domain binding; SH2 domain binding; antigen binding; glycoprotein binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol 3-kinase binding; protein C-terminus binding; protein binding; protein complex binding; protein kinase binding; protein serine/threonine phosphatase activity; protein serine/threonine phosphatase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine phosphatase activity

Reviews

04/24/2022

Good stability, long shelf-life.

10/29/2020

Consistent quality across batches.

03/11/2019

Pure product, reliable results.

Q&As

What role does LCK play in the development and maturation of T cells in the thymus?

LCK plays a vital role in the development and maturation of T cells in the thymus, especially during the processes of positive and negative selection, ensuring the generation of a functional and self-tolerant T-cell repertoire.

Can LCK activity be modulated by pharmacological agents?

Yes, LCK activity can be modulated by several pharmacological agents, including kinase inhibitors like dasatinib, which have been explored for therapeutic purposes in various diseases.

How is LCK activation regulated post-translationally?

LCK activation is regulated post-translationally through phosphorylation at specific tyrosine residues, with its activity being modulated by both activating and inhibitory phosphorylations.

What is the primary function of LCK in T-cell signaling?

LCK is a pivotal Src family kinase that plays a crucial role in initiating T-cell receptor (TCR) signaling by phosphorylating key tyrosines on the CD3 and ζ-chains of the TCR complex.

How does LCK interact with other kinases in T-cell signaling pathways?

LCK interacts with other kinases such as ZAP-70 and Fyn in T-cell signaling pathways, orchestrating a cascade of phosphorylation events that lead to T-cell activation.

Are there any known diseases or conditions associated with LCK dysregulation?

Dysregulation of LCK has been associated with various conditions, including autoimmune diseases, immunodeficiencies, and certain leukemias.

How does LCK contribute to T-cell receptor (TCR) complex formation and activation?

LCK contributes to TCR complex formation and activation by phosphorylating the immunoreceptor tyrosine-based activation motifs (ITAMs) on the TCR, facilitating the recruitment and activation of ZAP-70 kinase.