Recombinant Human MAP3K14 293 Cell Lysate

• Cat.No.: MAP3K14-4507HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for mitogen-activated protein kinase kinase kinase 14 (MAP3K14) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MAP3K14 mitogen-activated protein kinase kinase kinase 14 [ Homo sapiens ]
• Official Symbol: MAP3K14
• Synonyms: MAP3K14; mitogen-activated protein kinase kinase kinase 14; FTDCR1B; HS; HSNIK; NIK; serine/threonine protein kinase; NF-kappa-beta-inducing kinase; serine/threonine protein-kinase; serine/threonine-protein kinase NIK
• Gene ID: 9020
• mRNA Refseq: NM_003954
• Protein Refseq: NP_003945
• MIM: 604655
• UniProt ID: Q99558
• Chromosome Location: 17q21-q22
• Pathway: Adaptive Immune System, organism-specific biosystem; Alternative NF-kappaB pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem
• Function: ATP binding; MAP kinase kinase kinase activity; NF-kappaB-inducing kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity